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Internet-of-Things (IoT) approaches are continually introducing new sensors into the fields of agriculture and animal welfare. The application of multi-sensor data fusion to these domains remains a complex and open-ended challenge that defies straightforward optimization, often requiring iterative testing and refinement. To respond to this need, we have created a new open-source framework as well as a corresponding Python tool which we call the “Data Fusion Explorer (DFE)”. We demonstrated and evaluated the effectiveness of our proposed framework using four early-stage datasets from diverse disciplines, including animal/environmental tracking, agrarian monitoring, and food quality assessment. This included data across multiple common formats including single, array, and image data, as well as classification or regression and temporal or spatial distributions. We compared various pipeline schemes, such as low-level against mid-level fusion, or the placement of dimensional reduction. Based on their space and time complexities, we then highlighted how these pipelines may be used for different purposes depending on the given problem. As an example, we observed that early feature extraction reduced time and space complexity in agrarian data. Additionally, independent component analysis outperformed principal component analysis slightly in a sweet potato imaging dataset. Lastly, we benchmarked the DFE tool with respect to the Vanilla Python3 packages using our four datasets’ pipelines and observed a significant reduction, usually more than 50%, in coding requirements for users in almost every dataset, suggesting the usefulness of this package for interdisciplinary researchers in the field. 

We show that several models of interacting $XXZ$spin chains subject to boundary driving and dissipation possess a subtle kind of time-reversal symmetry, making their steady states exactly solvable. We focus on a model with a coherent boundary drive, showing that it exhibits a unique continuous dissipative phase transition as a function of the boundary drive amplitude. This transition has no analog in the bulk closed system or in incoherently driven models. We also show the steady-state magnetization exhibits a surprising fractal dependence on interaction strength, something previously associated with less easily measured infinite-temperature transport quantities (the Drude weight). Our exact solution also directly yields driven-dissipative double-chain models that have pure, entangled steady states that are current carrying. Published by the American Physical Society2025 

Guide dogs play a crucial role in enhancing independence and mobility for people with visual impairment, offering invaluable assistance in navigating daily tasks and environments. However, the extensive training required for these dogs is costly, resulting in a limited availability that does not meet the high demand for such skilled working animals. Towards optimizing the training process and to better understand the challenges these guide dogs may be experiencing in the field, we have created a multi-sensor smart collar system. In this study, we developed and compared two supervised machine learning methods to analyze the data acquired from these sensors. We found that the Convolutional Long Short-Term Memory (Conv-LSTM) network worked much more efficiently on subsampled data and Kernel Principal Component Analysis (KPCA) on interpolated data. Each attained approximately 40% accuracy on a 10-state system. Not needing training, KPCA is a much faster method, but not as efficient with larger datasets. Among various sensors on the collar system, we observed that the inertial measurement units account for the vast majority of predictability, and that the addition of environmental acoustic sensing data slightly improved performance in most datasets. We also created a lexicon of data patterns using an unsupervised autoencoder. We present several regions of relatively higher density in the latent variable space that correspond to more common patterns and our attempt to visualize these patterns. In this preliminary effort, we found that several test states could be combined into larger superstates to simplify the testing procedures. Additionally, environmental sensor data did not carry much weight, as air conditioning units maintained the testing room at standard conditions. 

Canine-assisted interactions (CAIs) have been explored to offer therapeutic benefits to human participants in various contexts, from addressing cancer-related fatigue to treating post-traumatic stress disorder. Despite their widespread adoption, there are still unresolved questions regarding the outcomes for both humans and animals involved in these interactions. Previous attempts to address these questions have suffered from core methodological weaknesses, especially due to absence of tools for an efficient objective evaluation and lack of focus on the canine perspective. In this article, we present a first-of-its-kind system and study to collect simultaneous and continuous physiological data from both of the CAI interactants. Motivated by our extensive field reviews and stakeholder feedback, this comprehensive wearable system is composed of custom-designed and commercially available sensor devices. We performed a repeated-measures pilot study, to combine data collected via this system with a novel dyadic behavioral coding method and short- and long-term surveys. We evaluated these multimodal data streams independently, and we further correlated the psychological, physiological, and behavioral metrics to better elucidate the outcomes and dynamics of CAIs. Confirming previous field results, human electrodermal activity is the measure most strongly distinguished between the dyads’ non-interaction and interaction periods. Valence, arousal, and the positive affect of the human participant significantly increased during interaction with the canine participant. Also, we observed in our pilot study that (a) the canine heart rate was more dynamic than the human’s during interactions, (b) the surveys proved to be the best indicator of the subjects’ affective state, and (c) the behavior coding approaches best tracked the bond quality between the interacting dyads. Notably, we found that most of the interaction sessions were characterized by extended neutral periods with some positive and negative peaks, where the bonded pairs might display decreased behavioral synchrony. We also present three new representations of the internal and overall dynamics of CAIs for adoption by the broader field. Lastly, this paper discusses ongoing options for further dyadic analysis, interspecies emotion prediction, integration of contextually relevant environmental data, and standardization of human–animal interaction equipment and analytical approaches. Altogether, this work takes a significant step forward on a promising path to our better understanding of how CAIs improve well-being and how interspecies psychophysiological states can be appropriately measured. 

Proteoforms, which arise from post-translational modifications, genetic polymorphisms and RNA splice variants, play a pivotal role as drivers in biology. Understanding proteoforms is essential to unravel the intricacies of biological systems and bridge the gap between genotypes and phenotypes. By analysing whole proteins without digestion, top-down proteomics (TDP) provides a holistic view of the proteome and can decipher protein function, uncover disease mechanisms and advance precision medicine. This Primer explores TDP, including the underlying principles, recent advances and an outlook on the future. The experimental section discusses instrumentation, sample preparation, intact protein separation, tandem mass spectrometry techniques and data collection. The results section looks at how to decipher raw data, visualize intact protein spectra and unravel data analysis. Additionally, proteoform identification, characterization and quantification are summarized, alongside approaches for statistical analysis. Various applications are described, including the human proteoform project and biomedical, biopharmaceutical and clinical sciences. These are complemented by discussions on measurement reproducibility, limitations and a forward-looking perspective that outlines areas where the field can advance, including potential future applications. 

Abstract Myosin functions as the “molecular motor” of the sarcomere and generates the contractile force necessary for cardiac muscle contraction. Myosin light chains 1 and 2 (MLC-1 and -2) play important functional roles in regulating the structure of the hexameric myosin molecule. Each of these light chains has an ‘atrial’ and ‘ventricular’ isoform, so called because they are believed to exhibit chamber-restricted expression in the heart. However, recently the chamber-specific expression of MLC isoforms in the human heart has been questioned. Herein, we analyzed the expression of MLC-1 and -2 atrial and ventricular isoforms in each of the four cardiac chambers in adult non-failing donor hearts using top-down mass spectrometry (MS)-based proteomics. Strikingly, we detected an isoform thought to be ventricular, MLC-2v, in the atria and confirmed the protein sequence using tandem MS (MS/MS). For the first time, a putative deamidation post-translation modification (PTM) located on MLC-2v in atrial tissue was localized to amino acid N13. MLC-1v and MLC-2a were the only MLC isoforms exhibiting chamber-restricted expression patterns across all donor hearts. Importantly, our results unambiguously show that MLC-1v, not MLC-2v, is ventricle-specific in adult human hearts. Overall, top-down proteomics allowed us an unbiased analysis of MLC isoform expression throughout the human heart, uncovering previously unexpected isoform expression patterns and PTMs. 

We present a mechanism that harnesses extremely weak Kerr-type nonlinearities in a single driven cavity to deterministically generate single-photon Fock states and more general photon-blockaded states. Our method is effective even for nonlinearities that are orders-of-magnitude smaller than photonic loss. It is also completely distinct from so-called unconventional photon blockade mechanisms, as the generated states are non-Gaussian, exhibit a sharp cutoff in their photon number distribution, and can be arbitrarily close to a single-photon Fock state. Our ideas require only standard linear and parametric drives and are hence compatible with a variety of different photonic platforms.